Last edited by Arashigor
Sunday, May 3, 2020 | History

7 edition of Protocols for oligonucleotide conjugates found in the catalog.

Protocols for oligonucleotide conjugates

synthesis and analytical techniques

by

  • 115 Want to read
  • 26 Currently reading

Published by Humana Press in Totowa, N.J .
Written in English

    Subjects:
  • Oligonucleotides -- Synthesis -- Laboratory manuals,
  • Oligonucleotides -- Analysis -- Laboratory manuals,
  • Oligonucleotides -- chemistry

  • Edition Notes

    Includes bibliographical references and index.

    Statementedited by Sudhir Agrawal ; foreword by H. Gobind Khorana.
    SeriesMethods in molecular biology ;, v. 26, Methods in molecular biology (Clifton, N.J.) ;, v. 26.
    ContributionsAgrawal, Sudhir.
    Classifications
    LC ClassificationsQP625.O47 P757 1994
    The Physical Object
    Paginationxiv, 377 p. :
    Number of Pages377
    ID Numbers
    Open LibraryOL1413806M
    ISBN 100896032523
    LC Control Number93023127

    Although no experimental details are presented, the chapter cites original source references. The next five chapters provide straightforward and detailed experimental protocols for synthesis of oligonucleotide conjugates, and may represent the major value Book Review Protocols for Oligonucleotide Conjugates. Protocols for Oligonucleotide Conjugates Synthesis and Analytical Techniques; Edited by S. Agarwal, The Humana Press, Totowa, NJ; xiv + pp. $ (pbk). ISBN o This monograph is a companion volume entitled ‘Protocols for Oligonucleotides and Analogs: Synthesis and Procedures’. The field of oligonucleotide therapeutics research is ripe with the prospect of new discoveries. In Therapeutic Oligonucleotides: Methods and Protocols, a selection of established and emerging methods for the application of oligonucleotides as therapeutics are presented, all providing the tools needed to inspire great changes in the field.


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Protocols for oligonucleotide conjugates Download PDF EPUB FB2

Protocols for Oligonucleotide Conjugates Synthesis and Analytical Techniques. Authors (view affiliations) Sudhir Agrawal. Protocols for Oligonucleotide Conjugates: Synthesis and Analytical Techniques (Methods in Molecular Biology) [Agrawal, Sudhir] on *FREE* shipping on qualifying offers.

Protocols for Oligonucleotide Conjugates: Synthesis and Analytical Techniques (Methods in Molecular Biology)Author: Sudhir Agrawal.

Protocols for Oligonucleotide Conjugates Synthesis and Analytical Techniques. Editors: Agrawal, Sudhir (Ed.) Free Preview. Buy this book eBook ,79 Protocols for Oligonucleotide Conjugates Book Subtitle Synthesis and Analytical Techniques Editors.

Sudhir Agrawal; Series Title Methods in Molecular Biology Series Volume Get this from a library. Protocols for oligonucleotide conjugates: synthesis and analytical techniques. [Sudhir Agrawal;] -- You will easily synthesize and analyze oligonucleotide conjugates by following the step-by-step protocols presented in this volume.

These. Get this from a library. Protocols for oligonucleotide conjugates: synthesis and analytical techniques. [Sudhir Agrawal;] -- Leading researchers provide the latest protocols for synthesizing oligonucleotide conjugates and explain the latest techniques for analyzing them.

These techniques are widely used by pharmaceutical. oligonucleotide synthesis Download oligonucleotide synthesis or read online books in PDF, EPUB, Tuebl, and Mobi Format.

A separate volume covers synthesis of oligonucleotide conjugates, along with most of the analytical techniques presently used for analysis of oligonucleotides. the book contains detailed protocols that make it suitable.

Protocols for oligonucleotide conjugates synthesis and analytical techniques In situ hybridization protocols (methods in molecular biology, Vol.

33) YAC protocols. You will easily synthesize and analyze oligonucleotide conjugates Protocols for oligonucleotide conjugates book following the step-by-step protocols presented in this volume.

These techniques are widely used by all molecular biologists and antisense researchers and find special application by pharmacologists working in new drug development and quality assurance assay.

About this book Introduction In Bioconjugation Protocols: Strategies and Methods, expert laboratorians update the classic methods and introduce valuable new approaches that go beyond basic conjugation techniques to include elements from advanced organic synthesis, molecular biology, and.

The field of oligonucleotide therapeutics research is ripe with the prospect of new discoveries. In Therapeutic Oligonucleotides: Methods and Protocols, a selection of established and emerging methods for the application of oligonucleotides as therapeutics are presented, all providing the tools needed to inspire great changes in the d into twenty-one chapters, this detailed volume Format: Hardcover.

Protecting groups in oligonucleotide synthesis / Etienne Sonveaux -- Incorporation of modified bases into oligonucleotides / Rich B.

Meyer -- Functionalization of oligonucleotides with amino groups and attachment of amino specific reporter groups / Sudhir Agrawal -- Functionalization of oligonucleotides by the incorporation of thio-specific Protocols for oligonucleotide conjugates book groups / Jacqueline A Fidanza, Hiroaki Ozaki.

The conjugates had a and a oligonucleotide/enzyme ratio, respectively, and were separated by ion-exchange chromatography.

Both conjugates were able to detect 7 amol of target DNA in 1 h. To date, several protocols for the chemical synthesis of oligonucleotide-peptide conjugates have been described (Lu et al.

; Aviñó et al. Conjugation of these large and functionalized. Protocols for oligonucleotide conjugates. Methods in molecular biology volume 26 edited by Sudhir Agrawal, Humana Press, US$ (xiv + pages) ISBN 0 3Author: Tom Brown. The present volume strives to bring the readers the most up-to-date information on the newest aspects of synthesis of oligo­ nucleotides and their analogs.

A separate volume covers synthesis of oligonucleotide conjugates, along with most of the analytical techniques presently used for analysis of oligonucleotides. Bioconjugate Techniques, Third Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics.

It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules.

The Paperback of the Protocols for Oligonucleotides and Analogs: Synthesis and Properties by Sudhir Agrawal at Barnes & Noble. FREE Shipping on $35 or. Get FREE SHIPPING on Orders of $35+ Customer information on COVID B&N Outlet Membership Educators Gift Cards Stores & Events Help.

Abstract: In this account we summarise recent studies on oligonucleotides and oligonucleotide derivatives and their utilisation in antigene, antisense and decoy approaches, with particular attention to peptide nucleic acids, locked nucleic acids and oligonucleotide conjugates, the Cited by: In this second edition of Bioconjugation Protocols: Strategies and Methods, expert researchers update the classic methods and introduce valuable new approaches that go beyond basic conjugation techniques to include elements from advanced organic synthesis, molecular biology, surface biotechnology, materials science, and nanobioscience.

Protocols for Oligonucleotide Conjugates. Synthesis and Analytical Techniques, (Methods in Molecular Biology/26), Edited by Sudhir Agrawal New Pharmacological Approaches to the Therapy of Depressive Disorders, (International Academy for Biomedical and Drug Research Volume 5) Edited by J.

Mendlewicz, N. Brunello, S. Langer, G. Racagni. The oligonucleotide conjugates were cleaved from the support and deprotected by 40% methylamine in the water at 65 °C for 15 min.

2′-O-TBDMS groups were removed upon treatment with a mixture of NMP/TEA3HF/TEA (//75) at 65 °C for : Mariya I. Meschaninova, Darya S. Novopashina, Olga A. Semikolenova, Vladimir N. Silnikov, Alya G. Abstract: In this account we summarise recent studies on oligonucleotides and oligonucleotide derivatives and their utilisation in antigene, antisense and decoy approaches, with particular attention to peptide nucleic acids, locked nucleic acids and oligonucleotide conjugates, the.

Similarly, oligonucleotide probes are often modified for detection with avidin or streptavidin conjugates. This chapter discusses the concept and use of the avidin–biotin interaction in bioconjugate techniques.

It also reviews the preparation of biotinylated molecules and avidin conjugates and provides the protocols. Protein Purification Protocols: Methods in Molecular Biology E d i t e d by Shawn D o o n a n. p p H u m a n a Press, T o t o w a, N e w Jersey. $ ISBN Protein Purification Protocols is an excellent collection of essential, time-tested recipes for fractionation and purification of.

Addition of small molecule Retro-1 has been described to enhance antisense and splice switching oligonucleotides. With the aim of assessing the effect of covalently linking Retro-1 to the biologically active oligonucleotide, three different derivatives of Retro-1 were prepared that incorporated a phosphoramidite group, a thiol or a 1,3-diene, : Jordi Agramunt, Enrique Pedroso, Silvia M.

Kreda, Rudolph L. Juliano, Anna Grandas. Interest in oligonucleotide synthesis has expanded dramatically since the publication of M. Gait's book in this series in The process has been automated, new reagents have been developed, and the range of uses for the compounds produced has increased and continues to grow.

ThisFile Size: KB. Protocols for Oligonucleotide Conjugates: Synthesis and Analytical Techniques (Methods in Molecular Biology) Book Download Online Psychosocial Nursing for General Patient Care Download Pdf Radar Handbook Download Pdf.

Sudhir Agrawal: free download. Ebooks library. On-line books store on Z-Library | B–OK. Download books for free. Find books. This book helps readers make: high activity antibody-enzymes conjugates, immunotoxins, immunogen complexes, liposome conjugates; as well as biotinylated molecules, avidin or streptavidin conjugates, colloidal gold labeled proteins, PEG or dextran complexes, labeled oligonucleotide probes, and fluorescently tagged or radiolabeled molecules.5/5(1).

This book helps readers make: high-activity antibody-enzyme conjugates, immunotoxins, immunogen complexes, liposome conjugates, as well as biotinylated molecules, avidin or streptavidin conjugates, colloidal gold-labeled proteins, PEG or dextran complexes, labeled oligonucleotide probes, and fluorescently tagged or radiolabeled molecules.

Current Protocols in Nucleic Acid Chemistry is the comprehensive resource for detailed protocols related to the synthesis, modification, and analysis of modified and unmodified nucleosides, nucleotides, oligonucleotides, and nucleic acids. Bioconjugate Techniques is the essential guide to the modification and crosslinking of biomolecules for use in research, diagnostics, and therapeutics.

It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents 5/5(1).

Oligonucleotide conjugates of small molecules are widely used in chemical biology and have found increasing interest in the context of DNA-encoded chemical libraries for drug discovery. Attachment of molecules to DNA bound to the solid support is an attractive small-molecule conjugation method that permits the use of organic solvents, rigorous.

Volume Oligonucleotide Synthesis ResearchGate. M E T H O D S I N M O L E C U L A R B I O L O G Y в„ў Oligonucleotide Synthesis Methods and Applications Edited by Piet Herdewijn Rega Institute, Medicinal Chemistry Lab, Oligonucleotide synthesis leads to the as they compete with the full-length product in some applications, This method results in a fairly pure.

Author(s): Agrawal,Sudhir Title(s): Protocols for oligonucleotide conjugates: synthesis and analytical techniques/ edited by Sudhir Agrawal ; foreword by H.

Gobind Khorana. Oligonucleotide conjugates of small molecules are widely used in chemical biology and have found increasing interest in the context of DNA-encoded chemical libraries for drug discovery.

Attachment of molecules to DNA bound to the solid support is an attractive small-molecule conjugation method that permits the use of organic solvents, rigorous reaction conditions, and simple by: 4. Cutting-edge and highly practical, Bioconjugation Protocols: Strategies and Methods, Second Edition offers both novice and experienced researchers access to the broad array of techniques needed to carry out the semisynthesis of functional biomolecular reagents and/or the biofunctionalization of surfaces and structures of unique interest for a.

A comprehensive review of contemporary antisense oligonucleotides drugs and therapeutic principles, methods, applications, and research Oligonucleotide-based drugs, in particular antisense oligonucleotides, are part of a growing number of pharmaceutical and biotech programs progressing to treat a wide range of indications including cancer, cardiovascular, neurodegenerative, neuromuscular, Format: Hardcover.

The comprehensive structure–activity relationships of triantennary GalNAc conjugated ASOs for enhancing potency via ASGR mediated delivery to hepatocytes is reported. Seventeen GalNAc clusters were assembled from six distinct scaffolds and attached to ASOs.

The resulting ASO conjugates were evaluated in ASGR binding assays, in primary hepatocytes, and in mice. N3-Cyanoethyl-dT. Synthesis of oligonucleotides on a large scale has become increasingly important in a variety of fields, including therapeutics and diagnostics.

2-Cyanoethyl (CE) phosphoramidites are produced on vast scales, are consequently inexpensive, and are by far the reagents of choice for oligonucleotide synthesis.

BioLegend offers an extensive variety of literature resources, including our fully detailed Catalog and Reference Manual, our beautifully illustrated posters, and other brochures and information sheets.

Each is provided free of charge. BioLegend develops and manufactures world-class, cutting-edge immunological reagents for biomedical research, offered at an outstanding value.Introduction. DNA-peptide conjugates have, in recent years, been identified as valuable tools in molecular biology.

In particular, they have become increasingly important due to the identification of peptides as viable carriers for enhancing the cell delivery of oligonucleotides. 1 Unfortunately, the hitherto cumbersome and inefficient methods of preparing DNA-peptide conjugates have hampered. Book Chapter.

The Assembly of Fluorescently Labeled Peptide-Oligonucleotide Conjugates via Orthogonal Ligation Strategies. John Karas, Bradley J Turner, Fazel Shabanpoor, T Yokota, R Maruyama EXON SKIPPING AND INCLUSION THERAPIES: METHODS AND PROTOCOLS | Methods in Molecular Biology | HUMANA PRESS INC | Published: DOI: / Cited by: 1.